TRNA (carboxymethyluridine34-5-O)-methyltransferase

TRNA (carboxymethyluridine³⁴-5-O)-methyltransferase (EC 2.1.1.229, ALKBH8, ABH8, Trm9, tRNA methyltransferase 9) is an enzyme with systematic name S-adenosyl-L-methionine:tRNA (carboxymethyluridine³⁴-5-O)-methyltransferase.^[1]^[2]^[3] This enzyme catalyses the following chemical reaction

S-adenosyl-L-methionine + carboxymethyluridine³⁴ in tRNA

\rightleftharpoons

S-adenosyl-L-homocysteine + 5-(2-methoxy-2-oxoethyl)uridine³⁴ in tRNA

The enzyme catalyses the posttranslational modification of uridine residues at the wobble position 34 of the anticodon loop of tRNA.

References

↑ Fu, D.; Brophy, J.A.; Chan, C.T.; Atmore, K.A.; Begley, U.; Paules, R.S.; Dedon, P.C.; Begley, T.J.; Samson, L.D. (2010). "Human AlkB homolog ABH8 Is a tRNA methyltransferase required for wobble uridine modification and DNA damage survival". Mol. Cell. Biol. 30 (10): 2449–2459. doi:10.1128/MCB.01604-09. PMID 20308323.
↑ Songe-Møller, L.; van den Born, E.; Leihne, V.; Vågbø, C.B.; Kristoffersen, T.; Krokan, H.E.; Kirpekar, F.; Falnes, P.Ø..; Klungland, A. (2010). "Mammalian ALKBH8 possesses tRNA methyltransferase activity required for the biogenesis of multiple wobble uridine modifications implicated in translational decoding". Mol. Cell. Biol. 30 (7): 1814–1827. doi:10.1128/MCB.01602-09. PMID 20123966.
↑ Kalhor, H.R.; Clarke, S. (2003). "Novel methyltransferase for modified uridine residues at the wobble position of tRNA". Mol. Cell. Biol. 23 (24): 9283–9292. doi:10.1128/mcb.23.24.9283-9292.2003. PMID 14645538.

External links

TRNA (carboxymethyluridine34-5-O)-methyltransferase at the US National Library of Medicine Medical Subject Headings (MeSH)

Transferase: one carbon transferases (EC 2.1)

2.1.1: Methyl-

N-	Histamine N-methyltransferase Phenylethanolamine N-methyltransferase Amine N-methyltransferase Phosphatidylethanolamine N-methyltransferase

O-	5-hydroxyindole-O-methyltransferase/Acetylserotonin O-methyltransferase Catechol-O-methyl transferase

Homocysteine	Betaine-homocysteine methyltransferase Homocysteine methyltransferase Methionine synthase

Other	Phosphatidyl ethanolamine methyltransferase DNMT3B Histone methyltransferase Thymidylate synthase DNA methyltransferase Thiopurine methyltransferase

2.1.2: Hydroxymethyl-,
Formyl- and Related

Hydroxymethyltransferase	Serine hydroxymethyltransferase 3-methyl-2-oxobutanoate hydroxymethyltransferase

Formyltransferase	Phosphoribosylglycinamide formyltransferase Inosine monophosphate synthase

Other	Glutamate formimidoyltransferase Aminomethyltransferase

2.1.3: Carboxy-
and Carbamoyl

Carboxy	methylmalonyl-CoA carboxytransferase

Carbamoyl	Aspartate carbamoyltransferase Ornithine carbamoyltransferase Oxamate carbamoyltransferase Putrescine carbamoyltransferase 3-hydroxymethylcephem carbamoyltransferase Lysine carbamoyltransferase N-acetylornithine carbamoyltransferase

2.1.4: Amidine

Arginine:glycine amidinotransferase

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list)

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