Protein N-terminal methyltransferase
| Protein N-terminal methyltransferase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 2.1.1.244 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
Protein N-terminal methyltransferase (EC 2.1.1.244, NMT1 (gene), METTL11A (gene)) is an enzyme with systematic name S-adenosyl-L-methionine:N-terminal-(A,P,S)PK-(protein) methyltransferase.[1][2] This enzyme catalyses the following chemical reaction
- (1) 3 S-adenosyl-L-methionine + N-terminal-(A,S)PK-[protein] 3 S-adenosyl-L-homocysteine + N-terminal-N,N,N-trimethyl-N-(A,S)PK-[protein] (overall reaction)
- (1a) S-adenosyl-L-methionine + N-terminal-(A,S)PK-[protein] S-adenosyl-L-homocysteine + N-terminal-N-methyl-N-(A,S)PK-[protein]
- (1b) S-adenosyl-L-methionine + N-terminal-N-methyl-N-(A,S)PK-[protein] S-adenosyl-L-homocysteine + N-terminal-N,N-dimethyl-N-(A,S)PK-[protein]
- (1c) S-adenosyl-L-methionine + N-terminal-N,N-dimethyl-N-(A,S)PK-serine-[protein] S-adenosyl-L-homocysteine + N-terminal-N,N,N-trimethyl-N-(A,S)PK-[protein]
- (2) 2 S-adenosyl-L-methionine + N-terminal-PPK-[protein] 2 S-adenosyl-L-homocysteine + N-terminal-N,N-dimethyl-N-PPK-[protein] (overall reaction)
- (2a) S-adenosyl-L-methionine + N-terminal-PPK-[protein] S-adenosyl-L-homocysteine + N-terminal-N-methyl-N-PPK-[protein]
- (2b) S-adenosyl-L-methionine + N-terminal-N-methyl-N-PPK-[protein] S-adenosyl-L-homocysteine + N-terminal-N,N-dimethyl-N-PPK-[protein]
This enzyme methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]-Pro-Lys.
References
- ↑ Webb, K.J.; Lipson, R.S.; Al-Hadid, Q.; Whitelegge, J.P.; Clarke, S.G. (2010). "Identification of protein N-terminal methyltransferases in yeast and humans". Biochemistry. 49: 5225–5235. doi:10.1021/bi100428x. PMID 20481588.
- ↑ Tooley, C.E.; Petkowski, J.J.; Muratore-Schroeder, T.L.; Balsbaugh, J.L.; Shabanowitz, J.; Sabat, M.; Minor, W.; Hunt, D.F.; Macara, I.G. (2010). "NRMT is an α-N-methyltransferase that methylates RCC1 and retinoblastoma protein". Nature. 466: 1125–1128. doi:10.1038/nature09343. PMID 20668449.
External links
- Protein N-terminal methyltransferase at the US National Library of Medicine Medical Subject Headings (MeSH)
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