PCBP1

Available structures

Ortholog search: PDBe RCSB

List of PDB id codes
1WVN, 1ZTG, 3VKE

Identifiers

Aliases

PCBP1, HEL-S-85, HNRPE1, HNRPX, hnRNP-E1, hnRNP-X, poly(rC) binding protein 1

External IDs

MGI: 1345635 HomoloGene: 68506 GeneCards: PCBP1

Gene ontology
Molecular function	• DNA binding • poly(A) RNA binding • protein binding • mRNA binding • RNA binding • nucleic acid binding • single-stranded DNA binding • cadherin binding involved in cell-cell adhesion • sequence-specific single stranded DNA binding • RNA polymerase II transcription factor activity, sequence-specific DNA binding
Cellular component	• cytoplasm • intracellular ribonucleoprotein complex • extracellular exosome • membrane • intracellular membrane-bounded organelle • nucleus • nucleoplasm • cell-cell adherens junction
Biological process	• mRNA splicing, via spliceosome • gene expression • cell-cell adhesion • viral RNA genome replication • positive regulation of transcription from RNA polymerase II promoter
Sources:Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

Entrez


5093


23983

Ensembl


ENSG00000169564


ENSMUSG00000051695

UniProt


Q15365


P60335

RefSeq (mRNA)


NM_006196


NM_011865

RefSeq (protein)


NP_006187.2


NP_035995.1

Location (UCSC)

Chr 2: 70.09 – 70.09 Mb

Chr 6: 86.52 – 86.53 Mb

PubMed search

^[1]

^[2]

Wikidata

View/Edit Human

View/Edit Mouse

Poly(rC)-binding protein 1 is a protein that in humans is encoded by the PCBP1 gene.^[3]

This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability.^[4]

References

↑ "Human PubMed Reference:".
↑ "Mouse PubMed Reference:".
↑ Tommerup N, Leffers H (May 1997). "Assignment of human KH-box-containing genes by in situ hybridization: HNRNPK maps to 9q21.32-q21.33, PCBP1 to 2p12-p13, and PCBP2 to 12q13.12-q13.13, distal to FRA12A". Genomics. 32 (2): 297–8. doi:10.1006/geno.1996.0121. PMID 8833161.
↑ "Entrez Gene: PCBP1 poly(rC) binding protein 1".

Kiledjian M, Wang X, Liebhaber SA (1995). "Identification of two KH domain proteins in the alpha-globin mRNP stability complex.". EMBO J. 14 (17): 4357–64. PMC 394520. PMID 7556077.
Leffers H, Dejgaard K, Celis JE (1995). "Characterisation of two major cellular poly(rC)-binding human proteins, each containing three K-homologous (KH) domains.". Eur. J. Biochem. 230 (2): 447–53. doi:10.1111/j.1432-1033.1995.tb20581.x. PMID 7607214.
Aasheim HC, Loukianova T, Deggerdal A, Smeland EB (1994). "Tissue specific expression and cDNA structure of a human transcript encoding a nucleic acid binding [oligo(dC)] protein related to the pre-mRNA binding protein K.". Nucleic Acids Res. 22 (6): 959–64. doi:10.1093/nar/22.6.959. PMC 307915. PMID 8152927.
Blyn LB, Swiderek KM, Richards O, et al. (1996). "Poly(rC) binding protein 2 binds to stem-loop IV of the poliovirus RNA 5' noncoding region: identification by automated liquid chromatography-tandem mass spectrometry.". Proc. Natl. Acad. Sci. U.S.A. 93 (20): 11115–20. doi:10.1073/pnas.93.20.11115. PMC 38293. PMID 8855318.
Kiledjian M, DeMaria CT, Brewer G, Novick K (1997). "Identification of AUF1 (heterogeneous nuclear ribonucleoprotein D) as a component of the alpha-globin mRNA stability complex.". Mol. Cell. Biol. 17 (8): 4870–6. PMC 232339. PMID 9234743.
Wang Z, Day N, Trifillis P, Kiledjian M (1999). "An mRNA stability complex functions with poly(A)-binding protein to stabilize mRNA in vitro.". Mol. Cell. Biol. 19 (7): 4552–60. PMC 84253. PMID 10373504.
Makeyev AV, Chkheidze AN, Liebhaber SA (1999). "A set of highly conserved RNA-binding proteins, alphaCP-1 and alphaCP-2, implicated in mRNA stabilization, are coexpressed from an intronless gene and its intron-containing paralog.". J. Biol. Chem. 274 (35): 24849–57. doi:10.1074/jbc.274.35.24849. PMID 10455157.
Makeyev AV, Liebhaber SA (2000). "Identification of two novel mammalian genes establishes a subfamily of KH-domain RNA-binding proteins.". Genomics. 67 (3): 301–16. doi:10.1006/geno.2000.6244. PMID 10936052.
Suzuki H, Fukunishi Y, Kagawa I, et al. (2001). "Protein-protein interaction panel using mouse full-length cDNAs.". Genome Res. 11 (10): 1758–65. doi:10.1101/gr.180101. PMC 311163. PMID 11591653.
Andersen JS, Lyon CE, Fox AH, et al. (2002). "Directed proteomic analysis of the human nucleolus.". Curr. Biol. 12 (1): 1–11. doi:10.1016/S0960-9822(01)00650-9. PMID 11790298.
Yeap BB, Voon DC, Vivian JP, et al. (2002). "Novel binding of HuR and poly(C)-binding protein to a conserved UC-rich motif within the 3'-untranslated region of the androgen receptor messenger RNA.". J. Biol. Chem. 277 (30): 27183–92. doi:10.1074/jbc.M202883200. PMID 12011088.
Walter BL, Parsley TB, Ehrenfeld E, Semler BL (2002). "Distinct poly(rC) binding protein KH domain determinants for poliovirus translation initiation and viral RNA replication.". J. Virol. 76 (23): 12008–22. doi:10.1128/JVI.76.23.12008-12022.2002. PMC 136902. PMID 12414943.
Giles KM, Daly JM, Beveridge DJ, et al. (2003). "The 3'-untranslated region of p21WAF1 mRNA is a composite cis-acting sequence bound by RNA-binding proteins from breast cancer cells, including HuR and poly(C)-binding protein.". J. Biol. Chem. 278 (5): 2937–46. doi:10.1074/jbc.M208439200. PMID 12431987.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Pickering BM, Mitchell SA, Evans JR, Willis AE (2003). "Polypyrimidine tract binding protein and poly r(C) binding protein 1 interact with the BAG-1 IRES and stimulate its activity in vitro and in vivo.". Nucleic Acids Res. 31 (2): 639–46. doi:10.1093/nar/gkg146. PMC 140511. PMID 12527772.
Pillai MR, Chacko P, Kesari LA, et al. (2003). "Expression of folate receptors and heterogeneous nuclear ribonucleoprotein E1 in women with human papillomavirus mediated transformation of cervical tissue to cancer.". J. Clin. Pathol. 56 (8): 569–74. doi:10.1136/jcp.56.8.569. PMC 1770025. PMID 12890803.
Adams DJ, Beveridge DJ, van der Weyden L, et al. (2004). "HADHB, HuR, and CP1 bind to the distal 3'-untranslated region of human renin mRNA and differentially modulate renin expression.". J. Biol. Chem. 278 (45): 44894–903. doi:10.1074/jbc.M307782200. PMID 12933794.
Evans JR, Mitchell SA, Spriggs KA, et al. (2003). "Members of the poly (rC) binding protein family stimulate the activity of the c-myc internal ribosome entry segment in vitro and in vivo.". Oncogene. 22 (39): 8012–20. doi:10.1038/sj.onc.1206645. PMID 12970749.
Chkheidze AN, Liebhaber SA (2003). "A novel set of nuclear localization signals determine distributions of the alphaCP RNA-binding proteins.". Mol. Cell. Biol. 23 (23): 8405–15. doi:10.1128/MCB.23.23.8405-8415.2003. PMC 262676. PMID 14612387.

PDB gallery

1wvn: Crsytal Structure of domain 3 of human alpha polyC binding protein

1ztg: human alpha polyC binding protein KH1

2axy: Crystal Structure of KH1 domain of human Poly(C)-binding protein-2 with C-rich strand of human telomeric DNA

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PCBP1

References

Further reading