Nuclear-inclusion-a endopeptidase

Nuclear-inclusion-a endopeptidase
Identifiers
EC number 3.4.22.44
CAS number 139946-51-3
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

Nuclear-inclusion-a endopeptidase (EC 3.4.22.44, potyvirus NIa protease) is a protease enzyme found in potyviruses.[1][2][3][4] This enzyme catalyses the following chemical reaction:

Hydrolyses glutaminyl bonds, and activity is further restricted by preferences for the amino acids in P6 - P1' that vary with the species of potyvirus.
e.g. Glu-Xaa-Xaa-Tyr-Xaa-Gln \ (Ser or Gly) for the enzyme from tobacco etch virus.

The enzyme is used encoded in vectors for the artificial expression of recombinant fusion proteins (see TEV protease).

References

  1. Fellers, J.P.; Collins, G.B.; Hunt, A.G. (1998). "The NIa-proteinase of different plant potyviruses provides specific resistance to viral infection". Crop Sci. 38: 1309–1319. doi:10.2135/cropsci1998.0011183x003800050030x.
  2. Kim, D.-H.; Choi, K.Y. (1998). "Potyvirus NIa protease". In Barrett, A.J.; Rawlings, N.D.; Woessner, J.F. Handbook of Proteolytic Enzymes. London: Academic Press. pp. 721–723.
  3. Takahashi, T.; Nakanishi, M.; Yao, Y.; Uyeda, I.; Serizawa, N. (1998). "Direct formation of human interleukin-11 by cis-acting system of plant virus protease in Escherichia coli". Biosci. Biotechnol. Biochem. 62: 953–958. doi:10.1271/bbb.62.953. PMID 9648226.
  4. Kim, D.H.; Hwang, D.C.; Kang, B.H.; Lew, J.; Han, J.S.; Song, B.O.D.; Choi, K.Y. (1996). "Effects of internal cleavages and mutations in the C-terminal region of NIa protease of turnip mosaic potyvirus on the catalytic activity". Virology. 226: 183–190. doi:10.1006/viro.1996.0645. PMID 8955037.
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