3-Ketosteroid 9alpha-monooxygenase

3-ketosteroid 9alpha-monooxygenase (EC 1.14.13.142, KshAB, 3-ketosteroid 9alpha-hydroxylase) is an enzyme with systematic name androsta-1,4-diene-3,17-dione,NADH:oxygen oxidoreductase (9alpha-hydroxylating).^[1]^[2]^[3] This enzyme catalyses the following chemical reaction

androsta-1,4-diene-3,17-dione + NADH + H⁺ + O₂

\rightleftharpoons

9alpha-hydroxyandrosta-1,4-diene-3,17-dione + NAD⁺ + H₂O

3-Ketosteroid 9alpha-monooxygenase is involved in the cholesterol degradation in several bacterial pathogens.

References

↑ Petrusma, M.; Dijkhuizen, L.; van der Geize, R. (2009). "Rhodococcus rhodochrous DSM 43269 3-ketosteroid 9α-hydroxylase, a two-component iron-sulfur-containing monooxygenase with subtle steroid substrate specificity". Appl. Environ. Microbiol. 75 (16): 5300–5307. doi:10.1128/AEM.00066-09. PMC 2725467. PMID 19561185.
↑ Capyk, J.K.; D'Angelo, I.; Strynadka, N.C.; Eltis, L.D. (2009). "Characterization of 3-ketosteroid 9α-hydroxylase, a Rieske oxygenase in the cholesterol degradation pathway of Mycobacterium tuberculosis". J. Biol. Chem. 284 (15): 9937–9946. doi:10.1074/jbc.M900719200. PMC 2665117. PMID 19234303.
↑ Capyk, J.K.; Casabon, I.; Gruninger, R.; Strynadka, N.C.; Eltis, L.D. (2011). "Activity of 3-ketosteroid 9α-hydroxylase (KshAB) indicates cholesterol side chain and ring degradation occur simultaneously in Mycobacterium tuberculosis". J. Biol. Chem. 286 (47): –. doi:10.1074/jbc.M111.289975. PMC 3220451. PMID 21987574.

External links

3-ketosteroid 9alpha-monooxygenase at the US National Library of Medicine Medical Subject Headings (MeSH)

Oxidoreductases: dioxygenases, including steroid hydroxylases (EC 1.14)

1.14.11: 2-oxoglutarate	Prolyl hydroxylase Lysyl hydroxylase

1.14.13: NADH or NADPH	Flavin-containing monooxygenase FMO1 FMO2 FMO3 FMO4 FMO5 Nitric oxide synthase NOS1 NOS2 NOS3 Cholesterol 7 alpha-hydroxylase Methane monooxygenase 3A4 Lanosterol 14 alpha-demethylase 24-hydroxycholesterol 7α-hydroxylase

1.14.14: reduced flavin or flavoprotein	19A1 2D6 2E1

1.14.15: reduced iron-sulfur protein	11B1 11B2 11A1

1.14.16: reduced pteridine (BH4 dependent)	Phenylalanine hydroxylase Tyrosine hydroxylase Tryptophan hydroxylase

1.14.17: reduced ascorbate	Dopamine beta-hydroxylase

1.14.18-19: other	Tyrosinase Stearoyl-CoA desaturase-1

1.14.99 - miscellaneous	Cyclooxygenase Heme oxygenase (HMOX1) Squalene monooxygenase 17A1 21A2

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list)

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