2-Succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase

2-Succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase

This image shows the two chains that come together to make up this enzyme
Identifiers
EC number 2.2.1.9
CAS number 122007-88-9
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

In enzymology, SEPHCHC synthase (EC EC 2.2.1.9), encoded by menD gene in E. coli, is an enzyme that catalyzes the second step of menaquinone (vitamin K2) biosynthesis. The two substrates of this enzyme are 2-oxoglutarate and isochorismate. The products of this enzyme are 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate and CO2. It belongs to the transferase family.

Classification

This enzyme belongs to a family of enzymes that transfer aldehyde or ketonic groups. To be specific, this enzyme belongs to the transketolase and transaldolase families. Common names for the enzyme are:

Reaction

In the biosynthesis of vitamin K, SEPHCHC is involved in the second step of the pathway.[1] The type of reaction is decarboxylating, and to have maximum activity, this enzymes uses the cofactor Mg2+, a magnesium ion.[2] In previous years, it was thought that this reaction led to SHCHC, the product MenH. After further research, we now know that this reaction is a new step in the pathway. The actual product of this enzyme can lose a pyruvate spontaneously.[3]

Step 2 in the biosynthesis of menaquinone

Structure

The structures reveal a stable dimer-of-dimers association in agreement with gel filtration and analytical studies confirm the classification of MenD in the pyruvate oxidase family. The active site is highly basic with a hydrophobic patch. These features match with the chemical properties of the substrates.[4]

Homologues

There are many similar structures MenD. Though it is commonly fouind in E. Coli, but can be found in other organisms as well. Bacillus subtilis and Mycobacterium tuberculosis are two homologues. All of the organisms share something in common, being that catalyze decarboxylation reactions.

References

This article is issued from Wikipedia - version of the 8/25/2016. The text is available under the Creative Commons Attribution/Share Alike but additional terms may apply for the media files.