2-deoxy-scyllo-inosose synthase

2-deoxy-scyllo-inosose synthase (EC 4.2.3.124, btrC (gene), neoC (gene), kanC (gene)) is an enzyme with systematic name D-glucose-6-phosphate phosphate-lyase (2-deoxy-scyllo-inosose-forming).^[1]^[2]^[3]^[4]^[5]^[6]^[7] This enzyme catalyses the following chemical reaction

D-glucose 6-phosphate

\rightleftharpoons

2-deoxy-L-scyllo-inosose + phosphate

This enzyme requires Co²⁺.

References

↑ Kudo, F.; Yamauchi, N.; Suzuki, R.; Kakinuma, K. (1997). "Kinetic isotope effect and reaction mechanism of 2-deoxy-scyllo-inosose synthase derived from butirosin-producing Bacillus circulans". J. Antibiot. (Tokyo). 50: 424–428. doi:10.7164/antibiotics.50.424. PMID 9207913.
↑ Kudo, F.; Hosomi, Y.; Tamegai, H.; Kakinuma, K. (1999). "Purification and characterization of 2-deoxy-scyllo-inosose synthase derived from Bacillus circulans. A crucial carbocyclization enzyme in the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics". J. Antibiot. (Tokyo). 52: 81–88. doi:10.7164/antibiotics.52.81. PMID 10344560.
↑ Kudo, F.; Tamegai, H.; Fujiwara, T.; Tagami, U.; Hirayama, K.; Kakinuma, K. (1999). "Molecular cloning of the gene for the key carbocycle-forming enzyme in the biosynthesis of 2-deoxystreptamine-containing aminocyclitol antibiotics and its comparison with dehydroquinate synthase". J. Antibiot. (Tokyo). 52: 559–571. doi:10.7164/antibiotics.52.559. PMID 10470681.
↑ Huang, Z.; Kakinuma, K.; Eguchi, T. (2005). "Stereospecificity of hydride transfer in NAD⁺-catalyzed 2-deoxy-scyllo-inosose synthase, the key enzyme in the biosynthesis of 2-deoxystreptamine-containing aminocyclitol antibiotics". Bioorg. Chem. 33: 82–89. doi:10.1016/j.bioorg.2004.09.002. PMID 15788164.
↑ Thuy, M.L.; Kharel, M.K.; Lamichhane, R.; Lee, H.C.; Suh, J.W.; Liou, K.; Sohng, J.K. (2005). "Expression of 2-deoxy-scyllo-inosose synthase (kanA) from kanamycin gene cluster in Streptomyces lividans". Biotechnol. Lett. 27: 465–470. doi:10.1007/s10529-005-2222-y. PMID 15928851.
↑ Tamegai, H.; Nango, E.; Koike-Takeshita, A.; Kudo, F.; Kakinuma, K. (2002). "Significance of the 20-kDa subunit of heterodimeric 2-deoxy-scyllo-inosose synthase for the biosynthesis of butirosin antibiotics in Bacillus circulans". Biosci. Biotechnol. Biochem. 66: 1538–1545. doi:10.1271/bbb.66.1538. PMID 12224638.
↑ Tamegai, H.; Sawada, H.; Nango, E.; Aoki, R.; Hirakawa, H.; Iino, T.; Eguchi, T. (2010). "Roles of a 20 kDa protein associated with a carbocycle-forming enzyme involved in aminoglycoside biosynthesis in primary and secondary metabolism". Biosci. Biotechnol. Biochem. 74: 1215–1219. doi:10.1271/bbb.90961. PMID 20530911.

External links

2-deoxy-scyllo-inosose synthase at the US National Library of Medicine Medical Subject Headings (MeSH)

Carbon-oxygen lyases (EC 4.2) (primarily dehydratases)

4.2.1: Hydro-Lyases	Carbonic anhydrase Fumarase Aconitase Enolase Alpha Enolase 2 Enoyl-CoA hydratase/3-Hydroxyacyl ACP dehydrase Methylglutaconyl-CoA hydratase Tryptophan synthase Cystathionine beta synthase Porphobilinogen synthase 3-Isopropylmalate dehydratase Urocanase Uroporphyrinogen III synthase Nitrile hydratase

4.2.2: Acting on polysaccharides	Hyaluronate lyase

4.2.3: Acting on phosphates	Threonine synthase

4.2.99: Other	Carboxymethyloxysuccinate lyase

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list)

This article is issued from Wikipedia - version of the 5/16/2016. The text is available under the Creative Commons Attribution/Share Alike but additional terms may apply for the media files.